As per the previous rounds in this competition the Boro team is likely to consist of a mixture of squad players and the youth team.
Stevenage beat London Colney (4-0) and Tring Athletic (4-0) to reach the quarter-finals whilst Hitchin have seen off Oxhey Jets (4-0) and Hoddeson Town (3-3, won 3-2 on pens) in their previous rounds.
The two teams last met in pre-season where Stevenage ran out 5-0 winners.
Kick-off at Top Field is 7.45pm and admission prices are £10 adults, £6 concessions and £2 for 16 and under.